GFAP Rabbit Polyclonal Antibody

Immunofluorescence analysis of Hela cell. 1. GFAP Polyclonal Antibody(green) was diluted at 1:200 (4° overnight). 2. Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3. DAPI(blue) 10min.

Immunofluorescence analysis of Hela cell. 1. GFAP Polyclonal Antibody(green) was diluted at 1:200 (4° overnight). 2. Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3. DAPI(blue) 10min.

Catalog Number:ABE5510
Conjugate:unconjugated
Size:100 ug
Concentration:1 mg/ml
Host:Rabbit
Isotype:IgG
Clone:Polyclonal
Immunogen:The antiserum was produced against synthesized peptide derived from human GFAP. AA range:11-60
Reactivity:Human;Rat;Mouse;
Applications:Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Molecular Weight:50kD
Purification:The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:GFAP; Glial fibrillary acidic protein; GFAP

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008],

Applications

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Immunogen

The antiserum was produced against synthesized peptide derived from human GFAP. AA range:11-60

Target Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008],

Synonyms:GFAP; Glial fibrillary acidic protein; GFAP

Storage

-20°C/1 year

Buffer

Liquid in PBS containing 50% glycerol, 0.5%BSAand0.02% sodium azide

Immunofluorescence analysis of Hela cell. 1. GFAP Polyclonal Antibody(green) was diluted at 1:200 (4° overnight). 2. Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3. DAPI(blue) 10min.

Western Blot analysis of RAT-MUSCLE cells using GFAP Polyclonal Antibody diluted at 1:2000

Western Blot analysis of RAW using GFAP Polyclonal Antibody diluted at 1:2000

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