GLI1
Western blot analysis of lysates from A-549 cells using GLI1 Rabbit at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 60s.
| Catalog Number: | AB23236 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human GLI1 (NP_005260.1). |
| Reactivity: | Hu, Ms, Rt |
| Applications: | WB, IHC, IF |
| Molecular Weight: | 160kDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
Applications
WB, IHC, IF
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human GLI1 (NP_005260.1).
