GLI3 Rabbit Monoclonal Antibody
Western blot analysis of extracts of HeLa cells, using Gli3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 180s.
| Catalog Number: | AB3300 |
| Conjugate: | Unconjugated |
| Size: | 100ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1301-1580 of human Gli3 (P10071). |
| Reactivity: | Hu,Ms, Rt |
| Applications: | WB, ELISA |
| Molecular Weight: | 190kDa |
| Purification: | Affinity Purified |
| Form: | Liquid |
This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B.
Applications
WB, ELISA
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1301-1580 of human Gli3 (P10071).
