Immunological Sciences

Glutamate receptor 1 Rabbit Polyclonal Antibody

Western Blot analysis of various cells using GluR-1 Polyclonal Antibody diluted at 1:500

Western Blot analysis of various cells using GluR-1 Polyclonal Antibody diluted at 1:500

Catalog Number:AB-J8091
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human GluR1. AA range:816-865
Reactivity:Human;Mouse;Rat
Applications:Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300 Immunofluorescence: 1/200 - 1/1000
Molecular Weight:95kDa
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:GRIA1; GLUH1; GLUR1; Glutamate receptor 1; GluR-1; AMPA-selective glutamate receptor 1; GluR-A; GluR-K1; Glutamate receptor ionotropic; AMPA 1; GluA1

Applications

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300 Immunofluorescence: 1/200 - 1/1000

Immunogen

The antiserum was produced against synthesized peptide derived from human GluR1. AA range:816-865

Target Background

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms:GRIA1; GLUH1; GLUR1; Glutamate receptor 1; GluR-1; AMPA-selective glutamate receptor 1; GluR-A; GluR-K1; Glutamate receptor ionotropic; AMPA 1; GluA1

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of various cells using GluR-1 Polyclonal Antibody diluted at 1:500

Western Blot analysis of HELA HEPG2 using GluR-1 Polyclonal Antibody diluted at 1:500

Immunofluorescence analysis of Hela cell. 1,GluR-1 Polyclonal Antibody(green) was diluted at 1:200(4°overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.

Immunofluorescence analysis of HeLa cells, using GluR1 Antibody. The picture on the right is blocked with the synthesized peptide.

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