Glutamate Transporter GLIA (GLAST /EAAT1)

Western blot analysis of extracts of various cell lines,
using EAAT1/EAAT1/SLC1A3 antibody
at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG
(H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s

Western blot analysis of extracts of various cell lines, using EAAT1/EAAT1/SLC1A3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s

Catalog Number:AB-84387
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids453-542 of human EAAT1/EAAT1/SLC1A3.
Reactivity:Hu, Ms, Rt
Applications:WB: 1:500 - 1:2000 IHC: 1:100 - 1:200
Molecular Weight:70kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:SLC1A3;EA6;EAAT1;GLAST;GLAST1

Applications

WB: 1:500 - 1:2000 IHC: 1:100 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids453-542 of human EAAT1/EAAT1/SLC1A3.

Target Background

This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.

Synonyms:SLC1A3;EA6;EAAT1;GLAST;GLAST1

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using EAAT1/EAAT1/SLC1A3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s

No documents available.