Gluthamine Synthetase (GS)

Western blot analysis of extracts of various cell lines, using Glutamine Synthetase (GLUL) antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.   Exposure time: 5s..

Western blot analysis of extracts of various cell lines, using Glutamine Synthetase (GLUL) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 5s..

Catalog Number:AB-83854
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-373 of human GS
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 -1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200
Molecular Weight:42kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:GS; GLNS; GS; PIG43; PIG59; glutamine synthetase

Applications

Western Blot: 1:500 -1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-373 of human GS

Target Background

The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. GS Polyclonal antibody is KO validated.

Synonyms:GS; GLNS; GS; PIG43; PIG59; glutamine synthetase

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.

Western blot analysis of extracts of various cell lines, using Glutamine Synthetase (GLUL) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 5s..

Western blot analysis of extracts from normal (control) and Glutamine Synthetase (GLUL) 293T cells, using Glutamine Synthetase (GLUL) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 5s.

Immunohistochemistry of paraffin embedded human esophageal using Glutamine Synthetase (Glutamine Synthetase (GLUL)) Rabbit pAb at dilution of 1:150 (40x lens).

Immunofluorescence analysis of MCF-7 cells using Glutamine Synthetase (Glutamine Synthetase (GLUL)) antibody Blue: DAPI for nuclear staining.

Review for Glutamine Synthetase (GLUL) Rabbit pAb Experiment Type Western blot SampleMCF-7

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