Immunological Sciences

GM130 Rabbit Polyclonal Antibody

Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, primary antibody was diluted at 1:1000, 4° over night, secondary antibody was diluted at 1:10000, 37° 1hour.

Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, primary antibody was diluted at 1:1000, 4° over night, secondary antibody was diluted at 1:10000, 37° 1hour.

Catalog Number:AB-84764
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Synthesized peptide derived from human GM130.
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100-1:200 ELISA: 1/10000
Molecular Weight:112-130kD
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:Golgin subfamily A member 2 (130 kDa cis-Golgi matrix protein;GM130;GM130 autoantigen;Golgin-95)

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100-1:200 ELISA: 1/10000

Immunogen

Synthesized peptide derived from human GM130.

Target Background

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.

Synonyms:Golgin subfamily A member 2 (130 kDa cis-Golgi matrix protein;GM130;GM130 autoantigen;Golgin-95)

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, primary antibody was diluted at 1:1000, 4° over night, secondary antibody was diluted at 1:10000, 37° 1hour.

Immunohistochemical analysis of paraffin-embedded human lung cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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