GOLPH3

Western blot analysis of extracts of various cell lines, using GOLPH3 antibody at 1:3000
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus. 
Exposure time: 15s.

Western blot analysis of extracts of various cell lines, using GOLPH3 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 15s.

Catalog Number:AB-84328
Conjugate:Unconjugated
Size:100ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-298 of human GOLPH3.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100 - 1:200 Immunofluorescence: 1:50 - 1:200
Molecular Weight:37kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:GOLPH3;GOPP1;GPP34;MIDAS;Vps74

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100 - 1:200 Immunofluorescence: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-298 of human GOLPH3.

Target Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.

Synonyms:GOLPH3;GOPP1;GPP34;MIDAS;Vps74

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using GOLPH3 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 15s.

Immunohistochemistry Paraffin-embedded human prostate cancer tissue stained for GOLPH3/MIDAS using at 1:100 dilution in immunohistochemical analysis.

Immunofluorescence analysis of L929 cells using GOLPH3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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