GRF01038

Recombinant Human IFN-gamma Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Catalog Number:	GRF01038
Size:	20 µg
Form:	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Synonyms:	IFNG;IFG;IFI

Recombinant Human IFN-gamma Protein

Target Background

Interferon-gamma (IFN-gamma ) is a secreted protein which belongs to the type II interferon family.Recombinant Human IFN-gamma is a 16.8 kDa protein containing 143 amino acid residues. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. Interferon-gamma is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway.

Synonyms:IFNG;IFG;IFI

Storage

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.

Recombinant Human IFN-gamma Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Recombinant Human IFN-gamma Protein is greater than 98.53% as determined by SEC-HPLC.

Immobilized Human FNGR1/CD119 at 1 μg/mL (100 μL/well) can bind Human IFNG with a linear range of 0.98-1.97ng/mL.

Recombinant Human IFN-gamma induces cytotoxicity in the HT-29 cells. The ED50 for this effect is 0.74-2.96 ng/mL, corresponding to a specific activity of 3.38×105~1.35×106 units/mg.

Flow cytometry: 1X106 A549 cells treated with IFN-γ (RP01038,1 ng/mL) were surface-stained with PE Rabbit IgG isotype control (A24172,5 μl/Test,left) or PE Rabbit anti-Human PD-L1/CD274 mAb (A26692,5 μl/Test,right)

Flow cytometry: 1X106 NCI-H460 cells treated with IFN-γ (RP01038,1 ng/mL) were surface-stained with PE Rabbit IgG isotype control (A24172,5 μl/Test,left) or PE Rabbit anti-Human PD-L1/CD274 mAb (A26692,5 μl/Test,right)

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