Immunological Sciences

GRF02806

The purity of Mouse Osteopontin/SPP1 is greater than 95% as determined by SEC-HPLC.

The purity of Mouse Osteopontin/SPP1 is greater than 95% as determined by SEC-HPLC.

Catalog Number:GRF02806
Size:20 ug
Form:lyophilized
Synonyms:Spp1; Eta-1; Op; Spp-1;Osteopontin; 2AR; Bone sialoprotein 1; Calcium oxalate crystal growth inhibitor protein; Early T-lymphocyte activation 1 protein; Minopontin; Secreted phosphoprotein 1; SPP-1
Species:HEK293 cells
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Recombinant Mouse Osteopontin/SPP-1 Protein

Target Background

Secreted phosphoprotein 1 (SPP1) expression in TAMs isolated from lung adenocarcinoma tissues and PMA-treated THP-1 cells were measured. Macrophage polarization was identified by flow cytometric analysis. Cell migration and apoptosis were assessed by Transwell migration assays and flow cytometric analysis, respectively. SPP1 is highly expressed in tumor tissues and TAMs isolated from patients with an advanced TNM stage, and also in PMA-treated THP-1 cells.

Synonyms:Spp1; Eta-1; Op; Spp-1;Osteopontin; 2AR; Bone sialoprotein 1; Calcium oxalate crystal growth inhibitor protein; Early T-lymphocyte activation 1 protein; Minopontin; Secreted phosphoprotein 1; SPP-1

Storage

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.

The purity of Mouse Osteopontin/SPP1 is greater than 95% as determined by SEC-HPLC.

The purity of Mouse Osteopontin/SPP1 is greater than 95% as determined by SEC-HPLC.

immobilized protein to support the adhesion of B16 cells.When 5×104 cells/well are added to a Recombinant Mouse (rm) Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is 21.0-83.9 ng/mL.

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