Immunological Sciences

GRP78 BiP Mouse Monoclonal Antibody

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Catalog Number:MABN85082
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Mouse
Isotype:Mouse IgG2b
Clone:Monoclonal
Immunogen:Purified recombinant human BiP/GRP78 protein expressed in E.coli.
Reactivity:Human,Mouse,Rat
Applications:WB 1:500-1:1000,ICC 1:50-1:200,IP 1:10-1:20
Molecular Weight:Calculated MW: 72 kDa; Observed MW: 78 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:HSPA5; GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP

Applications

WB 1:500-1:1000,ICC 1:50-1:200,IP 1:10-1:20

Immunogen

Purified recombinant human BiP/GRP78 protein expressed in E.coli.

Target Background

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).

Synonyms:HSPA5; GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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