GSK3β (phospho Ser9) rabbit Polyclonal Antibody

Western Blot analysis of Hela cells using Phospho-GSK3β (S9) Polyclonal Antibody diluted at 1:1000

Western Blot analysis of Hela cells using Phospho-GSK3β (S9) Polyclonal Antibody diluted at 1:1000

Catalog Number:ABP-1322
Conjugate:unconjugated
Size:100 ug
Concentration:1 mg/ml
Host:Rabbit
Isotype:IgG
Clone:Polyclonal
Immunogen:The antiserum was produced against synthesized peptide derived from human GSK3 beta around the phosphorylation site of Ser9. AA range:1-50
Reactivity:Human;Mouse;Rat;Drosophila
Applications:IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/5000. Not yet tested in other applications.
Molecular Weight:48kD
Purification:The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:liquid
Synonyms:GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009],

Applications

IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/5000. Not yet tested in other applications.

Immunogen

The antiserum was produced against synthesized peptide derived from human GSK3 beta around the phosphorylation site of Ser9. AA range:1-50

Target Background

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009],

Synonyms:GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5%BSAand0.02% sodium azide.

Western Blot analysis of Hela cells using Phospho-GSK3β (S9) Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from HeLa cells treated with EGF, using GSK3 beta (Phospho-Ser9) Antibody. The lane on the right is blocked with thephospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GSK3 beta (Phospho-Ser9) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of rat-spleen tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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