HE4/WFDC2 Rabbit Monoclonal Antibody

Western blot analysis of extracts of various cell lines, using HE4/WFDC2 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3min.

Western blot analysis of extracts of various cell lines, using HE4/WFDC2 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3min.

Catalog Number:MAB-95046
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:ARC1936
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 45-124 of human HE4/WFDC2.
Reactivity:Human, Mouse
Applications:Western Blot:    1:500 - 1:2000 Immunofluorescence:    1:50 - 1:200 Immunocytcohemistry:  1:50 - 1:200
Molecular Weight:22 kDa
Purification:Affinity purification
Form:Liquid
Synonyms:HE4; WAP5; EDDM4; dJ461P17.6; HE4/WFDC2

Applications

Western Blot:    1:500 - 1:2000 Immunofluorescence:    1:50 - 1:200 Immunocytcohemistry:  1:50 - 1:200

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 45-124 of human HE4/WFDC2.

Target Background

This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.

Synonyms:HE4; WAP5; EDDM4; dJ461P17.6; HE4/WFDC2

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using HE4/WFDC2 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3min.

Immunofluorescence analysis of NIH-3T3 cells using HE4/WFDC2 Rabbit mAb  at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using HE4/WFDC2 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

No documents available.