Immunological Sciences

HER2 / ERBB2

HeLa cells were subjected to SDS PAGE followed by western blot with (ERBB2 antibody) at dilution of 1:300

HeLa cells were subjected to SDS PAGE followed by western blot with (ERBB2 antibody) at dilution of 1:300

Catalog Number:AB-84340
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog(avian)
Reactivity:Hu, Ms
Applications:Western Blot: 1:1000-1:10000 Immunoprecipitation: 1:200-1:2000 Immunohistochemistry: 1:50-1:200
Molecular Weight:185kDa
Purification:Aff. Pur. ≥95% as determined by SDS-PAGE
Form:Liquid
Synonyms:CD340, ERBB2, HER 2, HER 2/neu, HER2, MLN 19, MLN19, NEU, NGL, p185, p185erbB2, Proto oncogene c ErbB 2, Proto oncogene Neu, TKR1

Applications

Western Blot: 1:1000-1:10000 Immunoprecipitation: 1:200-1:2000 Immunohistochemistry: 1:50-1:200

Immunogen

v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog(avian)

Target Background

HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor(EGF) receptor family of receptor tyrosine kinases. It has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas. This antibody raised against a synthesized peptide corresponding to 1237-1255aa of human HER2 recognizes the 185-kDa full-length glycosylated form and other lower molecular-mass forms of HER2, including the truncated form.

Synonyms:CD340, ERBB2, HER 2, HER 2/neu, HER2, MLN 19, MLN19, NEU, NGL, p185, p185erbB2, Proto oncogene c ErbB 2, Proto oncogene Neu, TKR1

Storage

T -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

HeLa cells were subjected to SDS PAGE followed by western blot with (ERBB2 antibody) at dilution of 1:300

Immunohistochemistry of paraffin-embedded human breast cancer using (ERBB2 antibody) at dilution of 1:50

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