Immunological Sciences

HIF-1alpha Rabbit Polyclonal Antibody

Western blot analysis of 1) Mouse Liver Tissue Lysate, 2)Rat Liver Tissue Lysate, 3) HepG2 Cell Lysate using HIF-1α Rabbit pAb diluted at 1:1000.

Western blot analysis of 1) Mouse Liver Tissue Lysate, 2)Rat Liver Tissue Lysate, 3) HepG2 Cell Lysate using HIF-1α Rabbit pAb diluted at 1:1000.

Catalog Number:AB-80484
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant Protein
Reactivity:Human, Mouse, Rat
Applications:WesternBlot:1/500-1000 Immunohistochemistry:1/100-200
Molecular Weight:Observed MW band: 90-130 kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:Hypoxia inducibel factor 1 alpha, HIF1A, ARNT, MOP1, PAS domaincontaining protein 8, PASD8

Applications

WesternBlot:1/500-1000 Immunohistochemistry:1/100-200

Immunogen

Recombinant Protein

Target Background

Hypoxia-inducible factor 1-alpha, also known as HIF-1-alpha, is a protein that in humans is encoded by the HIF1A gene. HIF-1-alpha found in mammalian cells growing at low oxygen concentrations. It plays an essential role in cellular and systemic responses to hypoxia.

Synonyms:Hypoxia inducibel factor 1 alpha, HIF1A, ARNT, MOP1, PAS domaincontaining protein 8, PASD8

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Western blot analysis of 1) Mouse Liver Tissue Lysate, 2)Rat Liver Tissue Lysate, 3) HepG2 Cell Lysate using HIF-1α Rabbit pAb diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HIF-1α Rabbit pAb diluted at 1:200

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