Immunological Sciences

His Tag (HRP Conj.)

Western blot analysis of recombinant His Tag protein using HRP-conj. Mouse anti His-Tag at dilution of 1:5000. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of recombinant His Tag protein using HRP-conj. Mouse anti His-Tag at dilution of 1:5000. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Catalog Number:MAB-91880HRP
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/mL
Host:Ms
Isotype:IgG1
Clone:B8
Immunogen:A synthetic peptide of His-Tag
Reactivity:All Species
Applications:• Western Blot (1:5000)
Purification:Affinity purification
Form:Liquid

Applications

• Western Blot (1:5000)

Immunogen

A synthetic peptide of His-Tag

Target Background

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-His antibodies.

Storage

Store at -20°C for 1 year. Store at -80°C for long term. Avoid repeated freeze and thaw cycles.

Buffer

PBS, 50% glycerol, pH7.3.

Western blot analysis of recombinant His Tag protein using HRP-conj. Mouse anti His-Tag at dilution of 1:5000. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

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