Immunological Sciences

HISTONE H2A Rabbit Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded  Human-uterus tissue. Histone H2A Polyclonal Antibody was diluted at 1:200(4°C,overnight).  Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min).Secondary antibody was diluted at 1:200

Immunohistochemical analysis of paraffin-embedded  Human-uterus tissue. Histone H2A Polyclonal Antibody was diluted at 1:200(4°C,overnight).  Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min).Secondary antibody was diluted at 1:200

Catalog Number:AB-E8591
Conjugate:unconjated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Polyclonal
Immunogen:Synthesized peptide derived from the Internal region of human Histone H2A. 20-50
Reactivity:Human, Mouse, Rat
Applications:Western Blot 1:500- 1:2000 IHC(p) 1:100-1:300 Elisa: 1:20000  
Molecular Weight:15kd
Purification:affinity purified
Form:Liquid
Synonyms:HIST1H2AG; H2AFP; HIST1H2AI; H2AFC; HIST1H2AK; H2AFD; HIST1H2AL; H2AFI; HIST1H2AM; H2AFN; Histone H2A type 1; H2A.1; Histone H2A/p

Applications

Western Blot 1:500- 1:2000 IHC(p) 1:100-1:300 Elisa: 1:20000  

Immunogen

Synthesized peptide derived from the Internal region of human Histone H2A. 20-50

Target Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones  (H2A, H2B, H3, and H4) form an octamer, around which ,approximately 146 bp of DNA is wrapped in repeating units,called nucleosomes. The linker histone, H1, interacts with  linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element.

Synonyms:HIST1H2AG; H2AFP; HIST1H2AI; H2AFC; HIST1H2AK; H2AFD; HIST1H2AL; H2AFI; HIST1H2AM; H2AFN; Histone H2A type 1; H2A.1; Histone H2A/p

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Immunohistochemical analysis of paraffin-embedded  Human-uterus tissue. Histone H2A Polyclonal Antibody was diluted at 1:200(4°C,overnight).  Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min).Secondary antibody was diluted at 1:200

Immunohistochemical analysis of  paraffin-embedded Mouse-lung tissue. 1,Histone H2A Polyclonal Antibody was diluted at 1:200(4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). Secondary antibody was diluted at 1:200(room temp., 30min). Negative control was used by secondary antibody only.

Western Blot analysis of HeLa cells using Histone H2A Polyclonal Antibody. Secondary antibody) was diluted at 1:20000 cells nucleus extracted

Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:100

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