Histone H3 Rabbit Monoclonal Antibody

Western blot analysis of lysates from Hela cells, using Histone H3 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.

Western blot analysis of lysates from Hela cells, using Histone H3 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.

Catalog Number:MABN21336
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG,Kappa
Clone:D1H2
Immunogen:A synthetic methylpeptide corresponding to residues surrounding Lys9 of human Histone H3
Reactivity:Human,Mouse,Rat
Applications:WB 1:5000-1:20000,IHC 1:5000-1:20000,ICC/IF 1:200-1:1000,ELISA 1:5000-1:20000,IP 1:50-1:200
Molecular Weight:Calculated MW:15kD;Observed MW:15kD
Purification:Affinity purification
Form:liquid
Synonyms:HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ;Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3

Cell localization:Nucleus.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

Applications

WB 1:5000-1:20000,IHC 1:5000-1:20000,ICC/IF 1:200-1:1000,ELISA 1:5000-1:20000,IP 1:50-1:200

Immunogen

A synthetic methylpeptide corresponding to residues surrounding Lys9 of human Histone H3

Target Background

Cell localization:Nucleus.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

Synonyms:HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ;Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%protective protein

Western blot analysis of lysates from Hela cells, using Histone H3 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.

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