Immunological Sciences

Histone H3 Recombinant Rabbit Monoclonal Antibody

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Catalog Number:MABN86363
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:A synthetic peptide of human Histone H3
Reactivity:Human, Mouse, Rat
Applications:WB 1:500-1:2000,IHC 1:2000-1:10000,ICC/IF 1:500-1:2000,FC 1:50-1:100
Molecular Weight:Calculated MW:15 kDa; Observed MW:17 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:H3/A; H3FA

Applications

WB 1:500-1:2000,IHC 1:2000-1:10000,ICC/IF 1:500-1:2000,FC 1:50-1:100

Immunogen

A synthetic peptide of human Histone H3

Target Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Synonyms:H3/A; H3FA

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

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