Immunological Sciences

HMGB1

Western blot on different Cells : Positive samples 293T, MCF7, Jurkat, Mouse spleen, Mouse lung, Mouse kidney, Rat lung Western blot analysis of extracts of various cell lines, using HMGB1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Kit Exposure time: 60s.

Western blot on different Cells : Positive samples 293T, MCF7, Jurkat, Mouse spleen, Mouse lung, Mouse kidney, Rat lung Western blot analysis of extracts of various cell lines, using HMGB1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Kit Exposure time: 60s.

Catalog Number:AB-83934
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1
Reactivity:Hu, Rt, Ms
Applications:Western Blot: 1:500 - 1: 2000 Immunohistochemistry: 1: 50 - 1: 200 Immunofluorescence: 1: 50 –1: 200
Molecular Weight:25kDa
Purification:Purified
Form:Liquid
Synonyms:HMGB1; HMG-1; HMG1; HMG3; SBP-1; high mobility group box 1

Applications

Western Blot: 1:500 - 1: 2000 Immunohistochemistry: 1: 50 - 1: 200 Immunofluorescence: 1: 50 –1: 200

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1

Target Background

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

Synonyms:HMGB1; HMG-1; HMG1; HMG3; SBP-1; high mobility group box 1

Storage

At +4°C for short term. For longer term store at -20°C, avoid repeat freeze-thaw cycles.

Buffer

Liquid in PBS containing 0.02% sodium azide, 50% glycerol, Ph7.3.

Western blot on different Cells : Positive samples 293T, MCF7, Jurkat, Mouse spleen, Mouse lung, Mouse kidney, Rat lung Western blot analysis of extracts of various cell lines, using HMGB1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Kit Exposure time: 60s.

Western blot analysis of extracts from normal (control) and HMGB1 on 293T cells, using HMGB1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded rat brain at 1:100

Immunohistochemistry of paraffin-embedded human breast cancer at 1:100

Immunofluorescence analysis of HeLa cells using HMGB1 antibody , at 1:100

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