Western blot analysis of extracts of various cell lines, 
using HSL antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG
 (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.   
Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using HSL antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

Catalog Number:AB-84408
Conjugate:Unconjugated
Size:100μg
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human LIPE.
Reactivity:Hu, Ms
Applications:Western Blot: 1:500 - 1:2000
Molecular Weight:81kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:LIPE;AOMS4;FPLD6;HSL;LHS

Applications

Western Blot: 1:500 - 1:2000

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human LIPE.

Target Background

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.

Synonyms:LIPE;AOMS4;FPLD6;HSL;LHS

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3

Western blot analysis of extracts of various cell lines, using HSL antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

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