Hsp27 Rabbit Monoclonal Antibody

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Catalog Number:MABN85678
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:A synthetic peptide of human Hsp27
Reactivity:Human,Mouse
Applications:WB 1:500-1:1000,IHC 1:50-1:100,ICC 1:50-1:200,IP 1:10-1:20
Molecular Weight:Calculated MW: 23 kDa; Observed MW: 27 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:HSPB1; HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27

Applications

WB 1:500-1:1000,IHC 1:50-1:100,ICC 1:50-1:200,IP 1:10-1:20

Immunogen

A synthetic peptide of human Hsp27

Target Background

Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels. In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway.

Synonyms:HSPB1; HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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