Immunological Sciences

HSP70 Mouse Monoclonal Antibody

Western blot analysis of extracts of various cell lines, using HSP70 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Western blot analysis of extracts of various cell lines, using HSP70 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Catalog Number:MAB-80111
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Mouse
Isotype:IgG1
Clone:SJ-70
Immunogen:A synthetic peptide of human HSP70
Reactivity:Human, Mouse, Rat
Applications:Western Blot 1:2000 - 1:5000
Molecular Weight:70kDa
Purification:Affinity Purified
Form:Liquid
Synonyms:HSPA1A;HEL-S-103;HSP70-1;HSP70-1A;HSP70.1;HSP70I;HSP72;HSPA1

Applications

Western Blot 1:2000 - 1:5000

Immunogen

A synthetic peptide of human HSP70

Target Background

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitinproteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Synonyms:HSPA1A;HEL-S-103;HSP70-1;HSP70-1A;HSP70.1;HSP70I;HSP72;HSPA1

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using HSP70 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

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