HSP90 alpha Rabbit Monoclonal Antibody

Western blot analysis of extracts of various cell lines, using Hsp90α Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.     
Exposure time: 3s.

Western blot analysis of extracts of various cell lines, using Hsp90α Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3s.

Catalog Number:MAB-80112
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:SJ-90
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Hsp90α
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:500 - 1:2000 Immunoprecipitation: 1:500 - 1:1000
Molecular Weight:90KDa
Purification:Purified
Form:Liquid
Synonyms:EL52; HEL-S-65p; HSP86; HSP89A; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; Hsp103; Hsp89; Hsp90; LAP-2; LAP2

Applications

Western Blot: 1:500 - 1:2000 Immunoprecipitation: 1:500 - 1:1000

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Hsp90α

Target Background

The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms:EL52; HEL-S-65p; HSP86; HSP89A; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; Hsp103; Hsp89; Hsp90; LAP-2; LAP2

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using Hsp90α Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3s.

Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug Hsp90α antibody. Western blot was performed from the immunoprecipitate using Hsp90α antibody at a dilution of 1:1000.

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