human IgG Mouse Monoclonal Antibody

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Catalog Number:MABN80501
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Mouse
Isotype:Mouse IgG1
Clone:Monoclonal
Immunogen:Human IgG was isolated from human sera and purified by chromatography.
Reactivity:Human
Applications:WB 1:500-1:2000,ELISA 1:5000-1:20000
Molecular Weight:50kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:human IgG

Applications

WB 1:500-1:2000,ELISA 1:5000-1:20000

Immunogen

Human IgG was isolated from human sera and purified by chromatography.

Target Background

Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.

Synonyms:human IgG

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Purified antibody in PBS with 0.05% sodium azide

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