IBA1 Rabbit Polyclonal Antibody

Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night

Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night

Catalog Number:AB-83747
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Synthesized peptide derived from part region of human protein
Reactivity:Human,Mouse,Rat
Applications:Western Blot: 1/500 - 1/2000Immunofluorescence: 1:100-300ELISA: 1/5000
Molecular Weight:16 kDa
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid

This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain.

Applications

Western Blot: 1/500 - 1/2000Immunofluorescence: 1:100-300ELISA: 1/5000

Immunogen

Synthesized peptide derived from part region of human protein

Target Background

This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain.

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night

Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min). 3, Picture B: DAPI(blue) 10min.

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