Immunological Sciences

IBA1

Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night

Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night

Catalog Number:AB-83747
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Synthesized peptide derived from part region of human protein
Reactivity:Hu, Ms, Rt
Applications:WB, IF
Molecular Weight:16 kDa
Purification:Aff. Pur.
Form:Liquid
Download Datasheet

This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain.

Applications

WB, IF

Immunogen

Synthesized peptide derived from part region of human protein

Target Background

This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain.

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night

Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min). 3, Picture B: DAPI(blue) 10min.

No documents available.