Iba1 Rabbit Monoclonal Antibody

Western blot detection of Iba1 in C6,3T3,Hela cell lysates using Iba1 antibody(1:1000 diluted).

Western blot detection of Iba1 in C6,3T3,Hela cell lysates using Iba1 antibody(1:1000 diluted).

Catalog Number:MABN86423
Conjugate:Unconjugated
Size:100 ug
Concentration:0.55mg/ml
Host:Rabbit
Isotype:IgG
Immunogen:A synthetic peptide of human Iba1
Reactivity:Human,Mouse,Rat
Applications:WB 1:500-1:2000,IHC 1:200-1:1000,ICC/IF 1:100-1:1000,FC 1:50-1:200
Molecular Weight:Observed MW:17 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:G1; Iba1; AIF-1; AI607846; D17H6S50E

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

Applications

WB 1:500-1:2000,IHC 1:200-1:1000,ICC/IF 1:100-1:1000,FC 1:50-1:200

Immunogen

A synthetic peptide of human Iba1

Target Background

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

Synonyms:G1; Iba1; AIF-1; AI607846; D17H6S50E

Storage

Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles.

Buffer

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% protective protein. Stable for 12 months from date of receipt.

Western blot detection of Iba1 in C6,3T3,Hela cell lysates using Iba1 antibody(1:1000 diluted).

Immunohistochemical analysis of paraffin-embedded human colon cancer using AMRe86423 antibody.

Western blot analysis of extracts from THP-1 cells using AMRe86423 at 1:1000.

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