IFN gamma

Western blot analysis of extracts of
various cell lines, using IFNG antibody
at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus 
Exposure time: 60s.

Western blot analysis of extracts of various cell lines, using IFNG antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 60s.

Catalog Number:AB-83768
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:poly
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 24-166 of human IFNG
Reactivity:Hu, Ms
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200
Molecular Weight:17-23kDa
Purification:Aff.Pur.
Form:Liquid
Synonyms:IFNG;IFG;IFI

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 24-166 of human IFNG

Target Background

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.

Synonyms:IFNG;IFG;IFI

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using IFNG antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 60s.

Immunohistochemistry of paraffin embedded human lung using IFNG antibody at dilution of 1:100 (40x lens).

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