Immunological Sciences

IGF 2

Western blot analysis of extracts of various cell lines, using IGF2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts of various cell lines, using IGF2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Catalog Number:AB-J1260
Conjugate:Unconjugated
Size:100μg
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-180 of human IGF2 (NP_000603.1).
Reactivity:Hu, Ms,
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:100
Molecular Weight:28kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:IGF2;C11orf43;GRDF;IGF-II;PP9974

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:100

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 25-180 of human IGF2 (NP_000603.1).

Target Background

This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' regionoverlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms:IGF2;C11orf43;GRDF;IGF-II;PP9974

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using IGF2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffinembedded human placenta using IGF2 Rabbit pAb at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A-549 cells using IGF2 antibody. Blue:DAPI for nuclear staining.

No documents available.