Immunological Sciences

IGF1R Rabbit Polyclonal Antibody

Western blot analysis of lysates from wild type (WT) and IGF1R knockdown (KD) 293T cells using [KDValidated] IGF1R Rabbit pAb at 1:3000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Western blot analysis of lysates from wild type (WT) and IGF1R knockdown (KD) 293T cells using [KDValidated] IGF1R Rabbit pAb at 1:3000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Catalog Number:AB-81364
Conjugate:Unconjugated
Size:100μg
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1201-1367 of human IGF1R.
Reactivity:Human,Mouse,Rat
Applications:Western Blot: 1:1000 - 1:5000
Molecular Weight:100kDa
Purification:Affinity purification
Form:Liquid
Synonyms:IGFR; CD221; IGFIR; JTK13; 1R

Applications

Western Blot: 1:1000 - 1:5000

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1201-1367 of human IGF1R.

Target Background

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Synonyms:IGFR; CD221; IGFIR; JTK13; 1R

Storage

At 4°C for one month. Or store frozen at -20°C for a longer time. Avoid freezing and thawing.

Buffer

PBS with 0.01% thimerosal,50% glycerol,pH7.3.

Western blot analysis of lysates from wild type (WT) and IGF1R knockdown (KD) 293T cells using [KDValidated] IGF1R Rabbit pAb at 1:3000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Western blot analysis of various lysates using [KD Validated] IGF1R Rabbit pAb at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

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