Immunological Sciences

IkB-alpha

Western blot analysis of lysates from MCF7 cells, treated with TNF-α, using IkappaB-alpha Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from MCF7 cells, treated with TNF-α, using IkappaB-alpha Antibody. The lane on the right is blocked with the synthesized peptide.

Catalog Number:AB-10165
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human IkappaB-alpha. AA range:15-64
Reactivity:Human,Mouse,Rat
Applications:Western Blot: 1/500 - 1/2000 Immunohistochemistry: 1/100 - 1/300 Immunofluorescence: 1/200 - 1/1000
Molecular Weight:40kDa
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:NFKBIA; IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3

Applications

Western Blot: 1/500 - 1/2000 Immunohistochemistry: 1/100 - 1/300 Immunofluorescence: 1/200 - 1/1000

Immunogen

The antiserum was produced against synthesized peptide derived from human IkappaB-alpha. AA range:15-64

Target Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.

Synonyms:NFKBIA; IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3

Storage

Store at -20℃. Avoid repeated freeze-thaw cycles

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western blot analysis of lysates from MCF7 cells, treated with TNF-α, using IkappaB-alpha Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin- embedded human breast carcinoma tissue, using IkappaB-alpha Antibody. The picture on the right is blocked with the synthesized peptide.

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