Immunological Sciences

IKK alpha Rabbit Polyclonal Antibody

Western Blot analysis of various cells using IKKα Polyclonal Antibody diluted at 1:1000

Western Blot analysis of various cells using IKKα Polyclonal Antibody diluted at 1:1000

Catalog Number:AB-81979
Conjugate:Unconjugated
Size:100μg
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human IKK-alpha. AA range:15-64
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1/500 - 1/2000 Immunohistochemistry: 1/100 - 1/300
Molecular Weight:85kD
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B

Applications

Western Blot: 1/500 - 1/2000 Immunohistochemistry: 1/100 - 1/300

Immunogen

The antiserum was produced against synthesized peptide derived from human IKK-alpha. AA range:15-64

Target Background

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

Synonyms:CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of various cells using IKKα Polyclonal Antibody diluted at 1:1000

Western Blot analysis of 293 cells using IKKα Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from 293 cells, treated with EGF, using IKK-alpha Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using IKK-alpha Antibody. The picture on the right is blocked with the synthesized peptide.

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