IL-1 beta Rabbit Monoclonal Antibody

Western blot analysis of IL-1 beta in various cell lines lysates using IL-1B antibody.

Western blot analysis of IL-1 beta in various cell lines lysates using IL-1B antibody.

Catalog Number:MABN03408
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:D3U3E
Immunogen:Recombinant protein of human IL1 beta
Reactivity:Human,Mouse,Rat
Applications:WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200
Molecular Weight:Calculated MW: 31 kDa; Observed MW: 17 kDa Mature Form, 31 kDa Precursor Form
Purification:Affinity Purification
Form:Liquid
Synonyms:IL1B; IL1F2; Interleukin-1 beta; IL-1 beta; Catabolin

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Applications

WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200

Immunogen

Recombinant protein of human IL1 beta

Target Background

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Synonyms:IL1B; IL1F2; Interleukin-1 beta; IL-1 beta; Catabolin

Storage

Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% protective protein and 0.02% sodium azide, pH 7.3.

Western blot analysis of IL-1 beta in various cell lines lysates using IL-1B antibody.

Western blot analysis of IL-1 beta in THP-1 lysates using IL-1B antibody.

Immunohistochemistry analysis of paraffin-embedded rat lung using IL-1B antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence analysis of IL-1 beta in THP-1 cells using IL-1B antibody,and DAPI (blue).

Immunofluorescence analysis of IL-1 beta in mouse lung using IL-1B antibody,and DAPI (blue).

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