Immunological Sciences

IL-10 Rabbit Polyclonal Antibody

Western Blot analysis of HepG2, Mouse liver cells using IL-10 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Western Blot analysis of HepG2, Mouse liver cells using IL-10 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Catalog Number:ABE3868
Conjugate:unconjugated
Size:100 ug
Concentration:1 mg/ml
Host:Rabbit
Isotype:IgG
Clone:Polyclonal
Immunogen:The antiserum was produced against synthesized peptide derived from the Internal region of human IL10. AA range:71-120
Reactivity:Human;Mouse;Rat
Applications:Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Molecular Weight:20kD
Purification:Affinity purification
Form:liquid
Synonyms:IL10; Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF
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The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Applications

Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.

Immunogen

The antiserum was produced against synthesized peptide derived from the Internal region of human IL10. AA range:71-120

Target Background

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011],

Synonyms:IL10; Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF

Storage

-20°C/1 year

Buffer

PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.

Western Blot analysis of HepG2, Mouse liver cells using IL-10 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.

Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:100

Western blot analysis of various lysates, using IL10 Rabbit pAb at 1:800 dilution. Raw264. 7 cells were treated with LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using IL10 Rabbit pAb ) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

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