IL-6 Rabbit Polyclonal Antibody

Western blot analysis of IL-6 in various cell lines lysates using IL-6 antibody.

Western blot analysis of IL-6 in various cell lines lysates using IL-6 antibody.

Catalog Number:ABN04151
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Recombinant protein of human IL6.
Reactivity:Human,Mouse,Rat
Applications:WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200
Molecular Weight:24 kDa
Purification:Affinity purification
Form:liquid
Synonyms:IL6; IFNB2; Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2

Interleukine 6 (IL-6) is a cytokine produced primarily by stimulated monocytes, fibroblasts and epithelial cells. It has a wide variety of biological functions through activating a cellsurface signaling assembly composed of IL6, IL6RA, and the shared signaling receptor gp130. The cytokine plays an essential role in the final differentiation of b-cells into Igsecreting cells, and induces myeloma, plasmacytoma growth, nerve cells differentiations. In addition, IL-6 is an important regulator of acute phase reaction.

Applications

WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200

Immunogen

Recombinant protein of human IL6.

Target Background

Interleukine 6 (IL-6) is a cytokine produced primarily by stimulated monocytes, fibroblasts and epithelial cells. It has a wide variety of biological functions through activating a cellsurface signaling assembly composed of IL6, IL6RA, and the shared signaling receptor gp130. The cytokine plays an essential role in the final differentiation of b-cells into Igsecreting cells, and induces myeloma, plasmacytoma growth, nerve cells differentiations. In addition, IL-6 is an important regulator of acute phase reaction.

Synonyms:IL6; IFNB2; Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% protective protein and 0.02% sodium azide, pH 7.3.

Western blot analysis of IL-6 in various cell lines lysates using IL-6 antibody.

Immunohistochemistry analysis of paraffin-embedded mouse spleen using IL-6 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence analysis of IL-6 in RAW264.7 cells using IL-6 antibody,and DAPI (blue).

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