Immunological Sciences

IL6 RAbbit Polyclonal Antibody

Western blot analysis of various lysates, using IL6 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

Western blot analysis of various lysates, using IL6 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

Catalog Number:AB-84785
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 30-212 of human IL6
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:500 - 1:1000 Immunofluorescence:1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200
Molecular Weight:24 kDa
Purification:Affinity purification
Form:Liquid
Synonyms:CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2; IL6

Applications

Western Blot: 1:500 - 1:1000 Immunofluorescence:1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 30-212 of human IL6

Target Background

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2).

Synonyms:CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2; IL6

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.05% proclin300,50% glycerol,pH7.3.

Western blot analysis of various lysates, using IL6 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

Immunofluorescence analysis of NIH/3T3 cells using IL6 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

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