Immunological Sciences

Insulin Rabbit Polyclonal Antibody

Western blot analysis of extracts of mouse pancreas, using Insulin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteInsulin: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Western blot analysis of extracts of mouse pancreas, using Insulin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteInsulin: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Catalog Number:AB-84377
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human INS.
Reactivity:Human, Mouse, Rat
Applications:<strong>Western Blot:                         </strong>1:500 - 1:2000 <strong>Immunohistochemistry:         </strong>1:50 - 1:200 <strong>Immunofluorescence:             </strong>1:50 - 1:200
Molecular Weight:12kDa
Purification:Affinity purification
Form:Liquid
Synonyms:IDDM;IDDM1;IDDM2;ILPR;IRDN;MODY10;INS;Insulin;insulin

Applications

<strong>Western Blot:                         </strong>1:500 - 1:2000 <strong>Immunohistochemistry:         </strong>1:50 - 1:200 <strong>Immunofluorescence:             </strong>1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human INS.

Target Background

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants.

Synonyms:IDDM;IDDM1;IDDM2;ILPR;IRDN;MODY10;INS;Insulin;insulin

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of mouse pancreas, using Insulin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteInsulin: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Immunohistochemistry of paraffin embedded rat pancreatic islet using Insulin antibody at dilution of 1:50 (40x lens).

Immunohistochemistry of paraffin embedded mouse pancreatic islet using Insulin antibody  at dilution of 1:50 (40x lens).

Immunofluorescence analysis of Rat pancreas using Insulin Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of Mouse pancreatic islet using Insulin Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

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