Immunological Sciences

Insulin Receptor Substrate 1

Western Blot analysis of HT29 cells using IRS-1 Polyclonal Antibody diluted at 1:1000

Western Blot analysis of HT29 cells using IRS-1 Polyclonal Antibody diluted at 1:1000

Catalog Number:AB-83501
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human IRS-1 AA range 603-652
Reactivity:Hu, Ms,Rt
Applications:WB 1:500 - 1:1000. IHC: 1:100-100 IF 1:100-200
Molecular Weight:170KD
Purification:Aff. Pur.
Form:Liquid
Synonyms:IRS-1

Applications

WB 1:500 - 1:1000. IHC: 1:100-100 IF 1:100-200

Immunogen

The antiserum was produced against synthesized peptide derived from human IRS-1 AA range 603-652

Target Background

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.

Synonyms:IRS-1

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Western Blot analysis of HT29 cells using IRS-1 Polyclonal Antibody diluted at 1:1000

Western Blot analysis of various cells using IRS-1 Polyclonal Antibody diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,IRS-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only

Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,IRS-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only

Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,IRS-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,IRS-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1,IRS-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of mouse-lung tissue. 1,IRS-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of human-lung tissue. 1,IRS-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Pict+Bure B:dapi C: merge A

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