Integrin B1

Western blot analysis of extracts of various cell lines, using ITGB1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus 
Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using ITGB1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 90s.

Catalog Number:AB-84115
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 600-700 of human ITGB1
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 -1:200 Immunofluorescence: 1:50 -1:200
Molecular Weight:100-135kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:ITGB1; CD29; FNRB; GPIIA; MDF2; MSK12; VLA-BET A; VLAB; integrin beta-1

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 -1:200 Immunofluorescence: 1:50 -1:200

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 600-700 of human ITGB1

Target Background

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.

Synonyms:ITGB1; CD29; FNRB; GPIIA; MDF2; MSK12; VLA-BET A; VLAB; integrin beta-1

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of various cell lines, using ITGB1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human gastric cancer using ITGB1 antibody at dilution of 1:100 (40x lens).

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