Immunological Sciences

CD25 Rabbit Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using CD25 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3s.

Western blot analysis of extracts of various cell lines, using CD25 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3s.

Catalog Number:AB-81365
Conjugate:Unconjugated
Size:100μg
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Immunogen A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CD25.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:1000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 Flow Cytometry: C 1:50 - 1:200
Molecular Weight:60KDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:p55; CD25; IL2R; IMD41; TCGFR; IDDM10

Applications

Western Blot: 1:500 - 1:1000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 Flow Cytometry: C 1:50 - 1:200

Immunogen

Immunogen A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CD25.

Target Background

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk.

Synonyms:p55; CD25; IL2R; IMD41; TCGFR; IDDM10

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.05% proclin300,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using CD25 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3s.

Immunohistochemistry of paraffinembedded human tonsil using CD25 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffinembedded rat spleen using CD25 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of rat spleen cells using CD25 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Flow cytometry: 293F Transfection cells(+) and 293F cells(-) were stained with Rabbit IgG isotype control (AC042, 10 ug/ml, blue line) or CD25 Rabbit pAb 2.5ug/ml orange line), followed by Goat anti-Rabbit pAb Alexa Fluor 647 (1:600 dilution) staining. Non-fluorescently stained Daudi cells was used as blank control (red line).

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