Immunological Sciences

JAK2 Rabbit Polyclonal Antibody

Western Blot analysis of HT29 cells using JAK2 Polyclonal Antibody diluted at 1:2000

Western Blot analysis of HT29 cells using JAK2 Polyclonal Antibody diluted at 1:2000

Catalog Number:AB-81994
Conjugate:Unconjugated
Size:100μg
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human JAK2. AA range:981-1030
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1/500 - 1/2000 Immunofluorescence: 1:50-200 Immunohistochemistry: 1/100 - 1/300
Molecular Weight:130kD
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:JAK2; Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2

Applications

Western Blot: 1/500 - 1/2000 Immunofluorescence: 1:50-200 Immunohistochemistry: 1/100 - 1/300

Immunogen

The antiserum was produced against synthesized peptide derived from human JAK2. AA range:981-1030

Target Background

This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis.

Synonyms:JAK2; Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of HT29 cells using JAK2 Polyclonal Antibody diluted at 1:2000

Western blot analysis of lysates from HT-29 cells, using JAK2 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,JAK2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,JAK2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,JAK2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of rat-lung tissue. 1,JAK2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-spleen tissue. 1,JAK2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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