Immunological Sciences

KCNH2 Rabbit Polyclonal Antibody

Western blot analysis of various lysates using KCNH2 Rabbit Polyclonal Ab at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 30s

Western blot analysis of various lysates using KCNH2 Rabbit Polyclonal Ab at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 30s

Catalog Number:AB-84820
Size:100 ug
Concentration:1 mg/ml
Host:Rabbit
Isotype:IgG
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 850-950 of human KCNH2
Reactivity:Human, Mouse
Applications:Western Blot  1:500 - 1:2000 IHC-P  1:50 - 1:200 IF/ICC  1:50 - 1:200 ELISA   1 μg/mL.
Molecular Weight:148 kDa
Purification:Affinity Purified
Form:Liquid
Synonyms:ERG1; HERG; LQT2; SQT1; ERG-1; H-ERG; HERG1; Kv11.1; KCNH2

Applications

Western Blot  1:500 - 1:2000 IHC-P  1:50 - 1:200 IF/ICC  1:50 - 1:200 ELISA   1 μg/mL.

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 850-950 of human KCNH2

Target Background

This gene encodes a component of a voltage-activated potassium channel found in cardiac muscle, nerve cells, and microglia. Four copies of this protein interact with one copy of the KCNE2 protein to form a functional potassium channel. Mutations in this gene can cause long QT syndrome type 2 (LQT2). Transcript variants encoding distinct isoforms have been identified.

Synonyms:ERG1; HERG; LQT2; SQT1; ERG-1; H-ERG; HERG1; Kv11.1; KCNH2

Storage

Store at -20℃. Avoid freeze / thaw cycles

Buffer

PBS with 0.05% proclin300,50% glycerol,pH7.3.

Western blot analysis of various lysates using KCNH2 Rabbit Polyclonal Ab at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 30s

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using KCNH2 Rabbit Polyclonal Ab at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using KCNH2 Rabbit Polyclonal Ab at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using KCNH2 Rabbit Polyclonal Ab at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunofluorescence analysis of Mouse kidney tissue using KCNH2 Rabbit Polyclonal Ab at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.

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