Immunological Sciences

KD-Validated AP1G1 Recombinant Rabbit Monoclonal Antibody

Western blotting analysis using AP1G1 antibody (KVAb01388). AP1G1 expression in wild type (WT) and AP1G1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AP1G1 antibody (KVAb01388, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (APS0635, 1:10,000) respectively.

Western blotting analysis using AP1G1 antibody (KVAb01388). AP1G1 expression in wild type (WT) and AP1G1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AP1G1 antibody (KVAb01388, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (APS0635, 1:10,000) respectively.

Catalog Number:MABN01388
Conjugate:Unconjugated
Size:100 ul
Concentration:1mg/ml
Host:Rabbit
Isotype:Rabbit IgG
Clone:188/Adaptin ɣ
Immunogen:A synthesized peptide derived from human AP1G1
Reactivity:Human,Mouse,Rat
Applications:WB 1:1,000-1:5,000; FC 1:200-1:2,000; ICC 1:100-1:1,000
Molecular Weight:91.4kDa
Purification:Affinity purification
Form:Liquid
Synonyms:AP1G1; Adaptor Related Protein Complex 1 Subunit Gamma 1; Gamma1-Adaptin; CLAPG1; ADTG; Clathrin Assembly Protein Complex 1 Gamma-1 Large Chain; Adaptor Related Protein Complex 1 Gamma 1 Subunit; Golgi Adaptor HA1/AP1 Adaptin Subunit Gamma-1; Adaptor Protein Complex AP-1 Subunit Gamma-1; AP-1 Complex Subunit Gamma-1; Clathrin-Associated/Assembly/Adaptor Protein, Large, Gamma 1; Clathrin Assembly Protein Complex 1 Gamma Large Chain; Adaptor-Related Protein Complex 1, Gamma 1 Subunit; Adapter-Related Protein Complex 1 Subunit Gamma-1; Adaptor-Related Protein Complex 1 Subunit Gamma-1; Golgi Adaptor HA1/AP1 Adaptin Gamma Subunit; Testicular Tissue Protein Li 21; Gamma Adaptin; USRISD

Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene.

Applications

WB 1:1,000-1:5,000; FC 1:200-1:2,000; ICC 1:100-1:1,000

Immunogen

A synthesized peptide derived from human AP1G1

Target Background

Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms:AP1G1; Adaptor Related Protein Complex 1 Subunit Gamma 1; Gamma1-Adaptin; CLAPG1; ADTG; Clathrin Assembly Protein Complex 1 Gamma-1 Large Chain; Adaptor Related Protein Complex 1 Gamma 1 Subunit; Golgi Adaptor HA1/AP1 Adaptin Subunit Gamma-1; Adaptor Protein Complex AP-1 Subunit Gamma-1; AP-1 Complex Subunit Gamma-1; Clathrin-Associated/Assembly/Adaptor Protein, Large, Gamma 1; Clathrin Assembly Protein Complex 1 Gamma Large Chain; Adaptor-Related Protein Complex 1, Gamma 1 Subunit; Adapter-Related Protein Complex 1 Subunit Gamma-1; Adaptor-Related Protein Complex 1 Subunit Gamma-1; Golgi Adaptor HA1/AP1 Adaptin Gamma Subunit; Testicular Tissue Protein Li 21; Gamma Adaptin; USRISD

Storage

Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles.

Buffer

Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.

Western blotting analysis using AP1G1 antibody (KVAb01388). AP1G1 expression in wild type (WT) and AP1G1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AP1G1 antibody (KVAb01388, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (APS0635, 1:10,000) respectively.

Western blotting analysis using AP1G1 antibody (KVAb01388). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AP1G1 antibody (KVAb01388, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (APS0635, 1:10,000) respectively.

Immunocytochemical staining of HepG2 cells with AP1G1 antibody (KVAb01388, 1:1,000). Nuclei were stained blue with DAPI; AP1G1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Flow cytometric analysis of AP1G1 expression in HepG2 cells using AP1G1 antibody 1:2,000. Green, isotype control; red, AP1G1.

No documents available.