Immunological Sciences

KD-Validated Beclin 1 Recombinant Rabbit Monoclonal Antibody

Western blotting analysis using beclin 1 antibody. Beclin 1 expression in wild type (WT) and beclin 1 (BECN1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with beclin 1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:10,000) respectively.

Western blotting analysis using beclin 1 antibody. Beclin 1 expression in wild type (WT) and beclin 1 (BECN1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with beclin 1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:10,000) respectively.

Catalog Number:MABN00772
Conjugate:Unconjugated
Size:100 ug
Host:Rabbit
Isotype:Rabbit IgG
Immunogen:Recombinant protein of human Beclin 1
Reactivity:Human,Mouse,Rat
Applications:WB 1:1,000-1:5,000; FC 1:200-1:2,000
Molecular Weight:60kDa
Purification:Affinity purification
Form:Liquid
Synonyms:BECN1; Beclin 1; VPS30; ATG6; Coiled-Coil Myosin-Like BCL2-Interacting Protein; Beclin 1, Autophagy Related; Beclin-1; Beclin 1 Beclin 1 Beclin1-Gamma; Protein GT197; Beclin1-Beta; Beclin1; GT197

This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.

Applications

WB 1:1,000-1:5,000; FC 1:200-1:2,000

Immunogen

Recombinant protein of human Beclin 1

Target Background

This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.

Synonyms:BECN1; Beclin 1; VPS30; ATG6; Coiled-Coil Myosin-Like BCL2-Interacting Protein; Beclin 1, Autophagy Related; Beclin-1; Beclin 1 Beclin 1 Beclin1-Gamma; Protein GT197; Beclin1-Beta; Beclin1; GT197

Storage

Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles.

Buffer

Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.

Western blotting analysis using beclin 1 antibody. Beclin 1 expression in wild type (WT) and beclin 1 (BECN1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with beclin 1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:10,000) respectively.

Western blotting analysis using beclin 1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with beclin 1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:10,000) respectively.

Validation of Beclin 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Beclin 1 antibody ( 1:2,000) and analyzed using BD flow cytometer.

No documents available.