KD-Validated LC3A/LC3B Rabbit Monoclonal Antibody
Western blotting analysis usingLC3A/LC3B antibody. LC3B expression in wild-type (WT) and LC3A/LC3B knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with LC3B antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:10,000) respectively.
| Catalog Number: | MABNK01267 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Host: | Rabbit |
| Isotype: | Rabbit IgG |
| Clone: | D3U4C |
| Immunogen: | A synthesized peptide derived from human LC3B |
| Reactivity: | Human,Mouse,Rat |
| Applications: | WB 1:1,000-1:5,000; FC 1:200-1:2,000; ICC 1:100-1:1,000; IHC-P 1:100-1:200 |
| Molecular Weight: | Calculated MW: 14.7kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | MAP1LC3BMicrotubule-Associated Proteins 1A/1B Light Chain 3B; Autophagy-Related Ubiquitin-Like Modifier LC3 B; MAP1 Light Chain 3-Like Protein 2; MAP1A/MAP1B Light Chain 3 B; MAP1A/MAP1B LC3 B; MAP1A/1BLC3; MAP1LC3B-A; MAP1ALC3; LC3B |
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]
Applications
WB 1:1,000-1:5,000; FC 1:200-1:2,000; ICC 1:100-1:1,000; IHC-P 1:100-1:200
Immunogen
A synthesized peptide derived from human LC3B
