Immunological Sciences

[KD Validated] LC3B Rabbit mAb

Western blot analysis of various lysates, using [KD Validated] LC3B Rabbit mAb at 1:1000 dilution. 293T, C6 and NIH/3T3 cells were treated by Chloroquine at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.

Western blot analysis of various lysates, using [KD Validated] LC3B Rabbit mAb at 1:1000 dilution. 293T, C6 and NIH/3T3 cells were treated by Chloroquine at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.

Catalog Number:AB19665
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:ARC0144
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 2-107 of human LC3B (Q9GZQ8).
Reactivity:Human,Mouse,Rat
Applications:WB,1:1000 - 1:4000 IHC-P,1:100 - 1:500 IF/ICC,1:200 - 1:800 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Molecular Weight:14kDa/16kDa
Purification:Affinity purification
Form:liquid

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Applications

WB,1:1000 - 1:4000 IHC-P,1:100 - 1:500 IF/ICC,1:200 - 1:800 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 2-107 of human LC3B (Q9GZQ8).

Target Background

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.09% sodium azid,0.05% BSA,50% glycerol,pH7.3.

Western blot analysis of various lysates, using [KD Validated] LC3B Rabbit mAb at 1:1000 dilution. 293T, C6 and NIH/3T3 cells were treated by Chloroquine at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.

Western blot analysis of lysates from wild type and LC3B knockdown 293T cells, using [KD Validated] LC3B Rabbit mAb at 1:1000 dilution. wild type and LC3B knockdown 293T cells were treated by Chloroquine at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.

Immunohistochemistry analysis of paraffin-embedded Human brain using [KD Validated] LC3B Rabbit mAb at dilution of 1:100 . High pressure antigen retrieval performed with 0.01M Citrate Bufferr prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brain using [KD Validated] LC3B Rabbit mAb at dilution of 1:100 . High pressure antigen retrieval performed with 0.01M Citrate Bufferr prior to IHC staining.

Confocal imaging of HeLa cells and HeLa cells using [KD Validated] LC3B Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG . DAPI was used for nuclear staining . Objective: 100x.

Confocal imaging of C6 cells and C6 cells using [KD Validated] LC3B Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG . DAPI was used for nuclear staining . Objective: 100x.

Confocal imaging of NIH/3T3 cells and NIH/3T3 cells using [KD Validated] LC3B Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG . DAPI was used for nuclear staining . Objective: 100x.

Immunoprecipitation analysis of 300 μg extracts from 293T cells using 3 μg [KD Validated] LC3B Rabbit mAb . Western blot was performed from the immunoprecipitate using LC3B antibody at a dilution of 1:1000.

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