Immunological Sciences

KD-Validated NR5A1/ SF-1 Mouse Monoclonal Antibody

Western blotting analysis using NR5A1 antibody. NR5A1 expression in wild-type (WT) and NR5A1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NR5A1 antibody 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody ( 1:10,000) respectively.

Western blotting analysis using NR5A1 antibody. NR5A1 expression in wild-type (WT) and NR5A1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NR5A1 antibody 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody ( 1:10,000) respectively.

Catalog Number:MABN01852
Conjugate:Unconjugated
Size:100 ug
Host:Mouse
Isotype:Mouse IgG1
Immunogen:A synthesized peptide derived from human NR5A1
Reactivity:Human
Applications:WB 1:400-1:2,000; ICC 1:100-1:1,000
Molecular Weight:Calculated MW: 51.6kDa
Purification:Affinity purification
Form:Liquid
Synonyms:NR5A1; Nuclear Receptor Subfamily 5 Group A Member 1; AD4BP; HSF-1; SF-1; SF1; Steroidogenic Factor 1; FTZF1; FTZ1; ELP; Steroid Hormone Receptor Ad4BP; Fushi Tarazu Factor Homolog 1; STF-1; Nuclear Receptor Subfamily 5, Group A, Member 1; Steroidogenic Factor 1 Nuclear Receptor; Adrenal 4 Binding Protein; Adrenal 4-Binding Protein; Nuclear Receptor AdBP4; Steroidogenic Factor-1; SPGF8; SRXX4; SRXY3; POF7

The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect. [provided by RefSeq, Jul 2008]

Applications

WB 1:400-1:2,000; ICC 1:100-1:1,000

Immunogen

A synthesized peptide derived from human NR5A1

Target Background

The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect. [provided by RefSeq, Jul 2008]

Synonyms:NR5A1; Nuclear Receptor Subfamily 5 Group A Member 1; AD4BP; HSF-1; SF-1; SF1; Steroidogenic Factor 1; FTZF1; FTZ1; ELP; Steroid Hormone Receptor Ad4BP; Fushi Tarazu Factor Homolog 1; STF-1; Nuclear Receptor Subfamily 5, Group A, Member 1; Steroidogenic Factor 1 Nuclear Receptor; Adrenal 4 Binding Protein; Adrenal 4-Binding Protein; Nuclear Receptor AdBP4; Steroidogenic Factor-1; SPGF8; SRXX4; SRXY3; POF7

Storage

Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles.

Buffer

Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.

Western blotting analysis using NR5A1 antibody. NR5A1 expression in wild-type (WT) and NR5A1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NR5A1 antibody 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody ( 1:10,000) respectively.

Western blotting analysis using NR5A1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NR5A1 antibody (1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (1:10,000) respectively.

Immunocytochemical staining of HepG2 cells with NR5A1 antibody ( 1:1,000). Nuclei were stained blue with DAPI; NR5A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

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