KD-Validated PPT1 Mouse Monoclonal Antibody
Western blotting analysis using PPT1 antibody. PPT1 expression in wild-type (WT) and PPT1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PPT1 antibody (1:2,500) and HRP-conjugated goat anti-mouse secondary antibody ( 1:10,000) respectively.
| Catalog Number: | MABN00047 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Host: | Mouse |
| Isotype: | Mouse IgG2b |
| Immunogen: | Recombinant protein of human PPT1 |
| Reactivity: | Human |
| Applications: | WB 1:500-1:2,500 |
| Molecular Weight: | Calculated MW: 34.2kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | PPT1; Palmitoyl-Protein Thioesterase 1; CLN1; INCL; PPT; Palmitoyl-Protein Hydrolase 1; EC 3.1.2.22; Ceroid-Palmitoyl-Palmitoyl-Protein Thioesterase 1; Ceroid-Lipofuscinosis, Neuronal 1, Infantile; PPT-1 |
The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2008]
Applications
WB 1:500-1:2,500
Immunogen
Recombinant protein of human PPT1
