[KD Validated] USF1 Rabbit mAb

Western blot analysis of lysates from PC-12 cells, using [KD Validated] USF1 Rabbit mAb  at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG   at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Western blot analysis of lysates from PC-12 cells, using [KD Validated] USF1 Rabbit mAb at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.

Catalog Number:AB23474
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:ARC58148
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human USF1. (NP_009053.1).
Reactivity:Human,Mouse,Rat
Applications:WB,1:2000 - 1:10000 IHC-P,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Molecular Weight:43kDa
Purification:Affinity purification
Form:liquid

This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21.

Applications

WB,1:2000 - 1:10000 IHC-P,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human USF1. (NP_009053.1).

Target Background

This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21.

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.

Western blot analysis of lysates from PC-12 cells, using [KD Validated] USF1 Rabbit mAb at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.

Western blot analysis of lysates from wild type and USF1 knockdown Hela cells, using [KD Validated] USF1 Rabbit mAb at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.

Immunohistochemistry analysis of paraffin-embedded Human spleen using USF1 Rabbit mAb at dilution of 1:900 . High pressure antigen retrieval performed with 0.01M Citrate Bufferr prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse lung using USF1 Rabbit mAb at dilution of 1:900 . High pressure antigen retrieval performed with 0.01M Citrate Bufferr prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat kidney using USF1 Rabbit mAb at dilution of 1:900 . High pressure antigen retrieval performed with 0.01M Citrate Bufferr prior to IHC staining.

Confocal imaging of paraffin-embedded Rat thymus using [KD Validated] USF1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG .DAPI was used for nuclear staining . Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer prior to IF staining.

Confocal imaging of paraffin-embedded Mouse brain using [KD Validated] USF1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG .DAPI was used for nuclear staining . Objective: 40x.

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