Ki-67

Western blot analysis of extracts of
NIH/3T3 cells, using Ki67 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus. 
Exposure time: 1s.

Western blot analysis of extracts of NIH/3T3 cells, using Ki67 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 1s.

Catalog Number:AB-84220
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant protein of human Ki67
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200
Molecular Weight:358kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:MKI67;KIA;MIB-;MIB-1;PPP1R105;marker of prolif eration Ki-67;Ki67

Applications

Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200

Immunogen

Recombinant protein of human Ki67

Target Background

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.

Synonyms:MKI67;KIA;MIB-;MIB-1;PPP1R105;marker of prolif eration Ki-67;Ki67

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of NIH/3T3 cells, using Ki67 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 1s.

Immunofluorescence analysis of C6 cells using Ki67 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using Ki67 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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